Flp-intm t-rextm 293 细胞系
WebBioz Stars score, Techniques, Protocol Conditions and more for Flp Intmt Rextm 293 Cells Hek 293t Hek Human Embryonic Kidney 293 Cells, supplied by Thermo Fisher. Data for … WebThe T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal …
Flp-intm t-rextm 293 细胞系
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Web丁香通为您提供FLPINTREX293商品详情介绍:价格:¥1 - 2000,货号:R78007,品牌:invitrogen,GIBCO,产地:美国,详见丁香通FLPINTREX293商品详情页; WebJul 2, 2012 · Als Kontrollen dienten mit dem Leervektor transfizierte und hCtr1 überexprimierende Flp-InTM T-RExTM-293-Zellen. Die rekombinanten hCtr2-Zellen erwiesen sich als signifikant weniger sensitiv gegenüber Cis- und Carboplatin als die Kontrollzellen. hCtr2 hatte hier also einen resistenzfördernden Effekt. Dies ist ein …
WebThe Flp-In ™ T‑REx -293 cell line is shipped on dry ice. Store in liquid nitrogen vapor-phase upon receipt. The Flp-In™ T‑REx™-293 cell line is supplied as one vial containing 1 × …
Web293t细胞由293细胞通过基因技术派生出的细胞系,是经过腺病毒e1a基因的转染,能表达sv40大t抗原,含有sv40复制起始点与启动子区。 许多真核表达载体如pcDNA3.1中含有SV40病毒的复制起始位点,可以在表达SV40病毒T抗原的细胞系中复制,从而提高外源基因 … WebOct 31, 2024 · HEK293细胞及其衍生株HEK293(human embryonic kidney 293),简称293细胞,来源于人胚胎肾细胞,由于在19号染色体整合了大约4.35 kb的腺病毒基因组片段,使其细胞周期以及细胞表型、核型等发生了 ... 293FTM细胞是由Flp-InTM T-RExTM 293细胞导入单嗜性(ecotropic)报告质粒和 ...
WebT-REx™ 细胞系可稳定表达四环素抑制子蛋白(表 1)。使用 T-REx™ 系统可以节省大量的时间和精力。通过使用阳性对照载体 pcDNA™4⁄TO⁄lacZ 进行瞬时转染,对 T-REx™ 细胞系进行功能检测。在用四环素诱导时,T-REx™ 细胞系在抑制状态下表现出极低的基础表达水平,表达水平较高(图 1)。
http://www.bio-thing.com/p118025 inbox mrspeterbrumberg2 gmai.comWebgenomic location. To generate these cell lines, the Flp-In ™ T-REx System involves the following major steps: 1. Independent integration of the following two plasmids into the … inbox moved in outlookWebMar 26, 2024 · Protocol 2 Protein complex purification from Flp-InTM T-RExTM 293 stably expressed FLAG tagged protein cell line Protocol 3 ChiP-Sequencing from Flp-InTM T-RExTM 293 stably expressed FLAG tagged protein cell lines Protocol 4 ChiP assay for endogenous gene Protocol 5 The cancer cell index, migration, and invasion assays Color … inbox msfWebFlp-In™系统使您可以稳定整合和表达感兴趣的基因,以提供单拷贝等基因细胞系。Flp-In™表达会在您选择的哺乳动物细胞系基因组中引入一个Flp重组靶标(FRT)位点。随后,包含您感兴趣的基因的表达载体会通过FRT位点的Flp重组酶介导DNA重组,整合入基因组。 in another world with my smartphone malWebSep 29, 2024 · 293FTM 细胞 是由 Flp-InTM T-RExTM 293 ... 总之, 293系列细胞经过长时间的改造与优化,已在多个领域实现了较多的应用,源井生物对于293与293T细胞也有丰富的基因编辑和细胞模型构建的经验,并拥有上千种293细胞的KO现货,覆盖超20种信号通 … in another world with my smartphone latinoWebATCC flp intm t rextm 293 cells Flp Intm T Rextm 293 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS … in another world with my smartphone lightWebThermo Fisher flp in t rex human embryonic kidney 293 hek293t cell line Flp In T Rex Human Embryonic Kidney 293 Hek293t Cell Line, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ... HEK293T-Flp-InTM T-RexTM (HEK293T) cells were maintained and cultured in DMEM media … inbox mozilla thunderbird email